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KMID : 0364820170530030163
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Korean Journal of Microbiology
2017 Volume.53 No. 3 p.163 ~ p.169
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Identification of Lactobacillus spp. associated with nematodes in peach farm soil
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Lee Woo-Hyun
Choi Jae-Im
Lee JIn-Il
Lee Won-Pyo
Yoon Sung-Sik
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Abstract
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Strains D4 and D5 were isolated from peach-rotten soil during the peach harvest season. The isolates were identified based on morphological and biochemical characterization, and identification was determined by 16S rRNA gene sequencing. Results showed that D4 has high similarity to Lactobacillus plantarum ATCC 14917 T and Lactobacillus pentosus ATCC 8041 T at 99.05% and 98.98%, respectively. D5 was also similar to Lactobacillus pentosus ATCC 8041 T and Lactobacillus plantarum ATCC 14917 T at 98.71% and 98.64%, respectively. In contrast, isolates showed differences in carbohydrate utilization in comparison to Lactobacillus plantarum ATCC 14917 T and Lactobacillus pentosus ATCC 8041 T . In view of this we performed VITEK MS matrix-assisted laser desorption ionization-time of flight mass spectrometry (MALDI-TOF MS) analysis, multiplex PCR fingerprinting, and random amplified polymorphic DNA (RAPD)- PCR to further confirm the identification of D4 and D5. The results of these analyses showed that both strains were most similar to Lactobacillus plantarum.
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KEYWORD
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Lactobacillus plantarum, MALDI-TOF MS, multiplex PCR, peach farm soil, RAPD-PCR
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